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| Catalog Number | Size | Price |
|---|---|---|
| LS-C26364-100 | 100 µg | $832 |
Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (C‑Terminus, IHC, WB) LS‑C26364
Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (C‑Terminus, IHC, WB) LS‑C26364
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Topoisomerase II Alpha antibody LS-C26364 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A) (C-Terminus). Validated for Flow, ICC, IHC, IP and WB.
Target
Human TOP2A / Topoisomerase II Alpha
Synonyms
TOP2A | DNA topoisomerase II, 170 kD | TOP2 | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Purification
Purified
Modifications
Unmodified
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Epitope
C-Terminus
Specificity
Recognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a carboxy terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.
Applications
- IHC
- IHC - Paraffin
- ICC (5 - 10 µg/ml)
- Western blot (1 - 10 µg/ml)
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10 ug/ml.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TOP2A / Topoisomerase II Alpha
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.312-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
