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Catalog Number Size Price
LS-C26364-100 100 µg $832 
Antibody

Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (C‑Terminus, IHC, WB) LS‑C26364

Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (C‑Terminus, IHC, WB) LS‑C26364

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$832
LS-C26364-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Topoisomerase II Alpha antibody LS-C26364 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A) (C-Terminus). Validated for Flow, ICC, IHC, IP and WB.
Target
Human TOP2A / Topoisomerase II Alpha
Synonyms
TOP2A | DNA topoisomerase II, 170 kD | TOP2 | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Epitope
C-Terminus
Specificity
Recognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a carboxy terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.
Applications
  • IHC
  • IHC - Paraffin
  • ICC (5 - 10 µg/ml)
  • Western blot (1 - 10 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10 ug/ml.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TOP2A / Topoisomerase II Alpha
P11388 NM_001067 NP_001058.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024