Human TLR4

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

HTA125

Unconjugated

Purified

Concentrated

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The HTA125 monoclonal antibody reacts preferentially, especially in flow cytometry, with human TLR4 that is associated with MD-2. HTA125 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to precipitate TLR4 (100 kD).

• Immunoprecipitation
• Flow Cytometry

The antibody can be used for flow cytometry, immuno precipitation and immunohistology on frozen sections. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production.

PBS, 0.02% Sodium Azide, 0.1% BSA

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00206 NCBI: NM_138554 O00206