Human TJP1 / ZO-1

Rabbit

Human, Mouse, Rat, Dog, Guinea pig (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

A 69 kDa fusion protein corresponding to aa 463-1109 of human ZO-1 cDNA. This sequence lies N-Terminus to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.

aa463-1109

Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen
• Western blot (0.5 - 2 µg/ml)
• Immunoprecipitation
• ELISA

Immunohistochemistry: LS-B2129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2129 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Immunoprecipitation: 2-5 ug/reaction.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07157 NCBI: NM_003257 NP_003248.3