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| Catalog Number | Size | Price |
|---|---|---|
| LS-B9774-300 | 300 µg (3 mg/ml) | $460 |
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PathPlus™ Polyclonal Goat anti‑Human TJP1 / ZO‑1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B9774
PathPlus™ Polyclonal Goat anti‑Human TJP1 / ZO‑1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B9774
Note: This antibody replaces LS-C204268
Antibody:
TJP1 / ZO-1 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TJP1 / ZO-1 Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
TJP1 (Tight junction protein-1, Zonula occludens-1, ZO-1) is a scaffold protein involved in tight junction assembly, anchoring and stabilization. It functions in cell-cell junction signal transduction and in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. TJP1 is upregulated in and drug-resistant multiple myeloma, and is associated with a higher risk subset of cancers. TJP1 has been found to be upregulated in drug-resistant tumors. In immunohistochemistry of normal tissue, TJP1 has membranous positivity in endothelial cells, glandular epithelia, renal glomeruli, sertoli cells, and is expressed in most tissues throughout the body.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; Oncoscience. 2017 Jul; 4(7-8): 79–94, PMID: 28966941
Target
Human TJP1 / ZO-1
Synonyms
TJP1 | ZO-1 | Zona occludens protein 1 | Zona occludens 1 | Zonula occludens protein 1 | Tight junction protein 1 | ZO1 | Tight junction protein ZO-1 | Zonula occludens 1 protein
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Purified recombinant peptide derived from within residues 1580 aa to the C-Terminus of human ZO-1 produced in E. coli.
Epitope
C-Terminus
Specificity
Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- IHC - Frozen (1:50 - 1:200)
- Immunofluorescence (1:50 - 1:250)
- Western blot (1:500 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
PBS, 0.05% Sodium Azide, 20% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TJP1 / ZO-1
Validation
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Stratum Basale: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin, Stratum Basale: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney, Positive Signal in Collecting Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney, Positive Signal in Collecting Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Positive signal in Sertoli Cell Membrane: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis, Positive signal in Sertoli Cell Membrane: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ TJP1 / ZO-1 Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western LS-B9774 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (3)
Inhibition of Retinal Ganglion Cell Loss By a Novel ROCK Inhibitor (E212) in Ischemic Optic Nerve Injury Via Antioxidative and Anti-Inflammatory Actions. Yao-Tseng Wen, Ching-Wen Huang, Chih-Peng Liu, Chih-Hung Chen, Chia-Mu Tu, Chrong-Shiong Hwang, Yi-Hsun Chen, Wan-Ru Chen, Keh-Liang Lin, Yu-Chieh Ho, Ta-Ching Chen , Rong-Kung Tsai. The Breast : official journal of the European Society of Mastology. 2021 May;62:21.
AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration. Kiyoharu J Miyagishima , Ruchi Sharma , Malika Nimmagadda , Katharina Clore-Gronenborn, Zoya Qureshy, Davide Ortolan, Devika Bose, Mitra Farnoodian, Congxiao Zhang, Andrew Fausey, Yuri V Sergeev, Mones Abu-Asab, Bokkyoo Jun, Khanh V Do, Marie-Audrey Kautzman Guerin, Jorgelina Calandria, Aman George, Bin Guan, Qin Wan, Rachel C Sharp, Catherine Cukras, Paul A Sieving, Robert B Hufnagel, Nicolas G Bazan, Kathleen Boesze-Battaglia, Sheldon Miller, Kapil Bharti. Communications biology. 2021 December;4:1360.
Induction of Human Umbilical Mesenchymal Stem Cell Differentiation Into Retinal Pigment Epithelial Cells Using a Transwell-Based Co-culture System. Yu-Hsun Chang, V Bharath Kumar, Yao-Tseng Wen, Chih-Yang Huang, Rong-Kung Tsai, Dah-Ching Ding. Cell transplantation. 2022 March;31:9636897221085901.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
