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Catalog Number Size Price
LS-C330808-20 20 µl $275 
LS-C330808-100 100 µl $389 
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat kidney using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibodyat dilution of 1:100 (40x lens).
SPHK / SPHK1 Antibody - Immunofluorescence analysis of HeLa cells.
SPHK / SPHK1 Antibody - Western blot analysis of extracts of various cell lines, using SPHK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat kidney using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibodyat dilution of 1:200 (40x lens).
SPHK / SPHK1 Antibody - Immunohistochemistry of paraffin-embedded rat liver using SPHK1 Antibodyat dilution of 1:100 (40x lens).
SPHK / SPHK1 Antibody - Immunofluorescence analysis of HeLa cells.
SPHK / SPHK1 Antibody - Western blot analysis of extracts of various cell lines, using SPHK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (IHC, IF, WB) LS‑C330808

Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (IHC, IF, WB) LS‑C330808

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C330808-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SPHK1 antibody LS-C330808 is an unconjugated rabbit polyclonal antibody to SPHK1 (SPHK) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-384 of human SPHK1 (NP_001136074.1). TLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
Specificity
Human SPHK / SPHK1
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 42kDa/43kDa/51kDa, while the observed MW by Western blot was 55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPHK / SPHK1
Q9NYA1 NM_021972 NP_068807.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024