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Catalog Number Size Price
LS-A3102-50 50 µg (1 mg/ml) $460 
SPHK / SPHK1 Antibody - Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC‑plus™ Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (N‑Terminus, IHC) LS‑A3102

IHC‑plus™ Polyclonal Rabbit anti‑Human SPHK / SPHK1 Antibody (N‑Terminus, IHC) LS‑A3102

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-A3102-50

Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SPHK / SPHK1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SPHK1 antibody LS-A3102 is an unconjugated rabbit polyclonal antibody to human SPHK1 (SPHK) (N-Terminus). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from N-Terminus of human SPHK1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (93%); Bat, Pig (87%); Mouse, Rat, Hamster (80%).
Epitope
N-Terminus
Specificity
Human SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPHK / SPHK1
Q9NYA1 NM_021972 NP_068807.2

Validation

SPHK / SPHK1 Antibody - Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) for IHC LS-A3102 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024