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Catalog Number Size Price
LS-C87246-100 100 µg (0.5 mg/ml) $503 
SPHK / SPHK1 Antibody - Antibody (2 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Polyclonal Goat anti‑Human SPHK / SPHK1 Antibody (aa176‑189, WB) LS‑C87246

Polyclonal Goat anti‑Human SPHK / SPHK1 Antibody (aa176‑189, WB) LS‑C87246

Antibody:
SPHK / SPHK1 Goat anti-Human Polyclonal (aa176-189) Antibody
Application:
WB, IP, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C87246-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SPHK / SPHK1 Goat anti-Human Polyclonal (aa176-189) Antibody
Application:
WB, IP, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
SPHK1 antibody LS-C87246 is an unconjugated goat polyclonal antibody to SPHK1 (SPHK) (aa176-189) from human. It is reactive with human, mouse, hamster and other species. Validated for IP, Peptide-ELISA and WB.
Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Host
Goat
Reactivity
Human, Monkey, Mouse, Hamster (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2NP_892010.2NP_001136073.1.
Epitope
aa176-189
Specificity
Human SPHK1. This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
Applications
  • Western blot (2 - 3 µg/ml)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 40 kD band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kD according to NP_068807.2). Recommended concentration: 2-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPHK / SPHK1
Q9NYA1 NM_021972 NP_068807.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024