Target
Human SPHK / SPHK1
Synonyms
SPHK1 | SK1 | Sphingosine kinase 1 | SPHK | SK 1 | SPK 1 | Sphingosine kinase type 1 | SPK
Reactivity
Human, Monkey, Mouse, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2NP_892010.2NP_001136073.1.
Specificity
Human SPHK1. This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
Applications
- Western blot (2 - 3 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 40 kD band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kD according to NP_068807.2). Recommended concentration: 2-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee