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Catalog Number Size Price
LS-C680939-50 50 µg (1 mg/ml) $294 
LS-C680939-100 100 µg (3.68 mg/ml) $360 
PVRL4 / Nectin 4 Antibody - Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
PVRL4 / Nectin 4 Antibody - Immunofluorescence staining of MCF-7 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
PVRL4 / Nectin 4 Antibody - Western Blot Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate All Lanes: NECTIN4 antibody at 5.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 25 KDa Observed band size: 35 KDa
PVRL4 / Nectin 4 Antibody - Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
PVRL4 / Nectin 4 Antibody - Immunofluorescence staining of MCF-7 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
PVRL4 / Nectin 4 Antibody - Western Blot Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate All Lanes: NECTIN4 antibody at 5.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 25 KDa Observed band size: 35 KDa
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Polyclonal Rabbit anti‑Human PVRL4 / Nectin 4 Antibody (IHC, IF, WB) LS‑C680939

Polyclonal Rabbit anti‑Human PVRL4 / Nectin 4 Antibody (IHC, IF, WB) LS‑C680939

Antibody:
PVRL4 / Nectin 4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C680939-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PVRL4 / Nectin 4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Nectin 4 antibody LS-C680939 is an unconjugated rabbit polyclonal antibody to Nectin 4 (PVRL4) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human PVRL4 / Nectin 4
Synonyms
PVRL4 | EDSS1 | Nectin 4 | Ig superfamily receptor LNIR | Poliovirus receptor-like 4 | LNIR | Nectin-4 | Poliovirus receptor-related 4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Nectin-4 protein (91-199AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PVRL4 / Nectin 4
Q96NY8 NM_030916 NP_112178.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024