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Catalog Number Size Price
LS-B1889-50 50 µl $440 
Prohibitin 2 / PHB2 Antibody - Anti-PHB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Prohibitin 2 / PHB2 Antibody - Immunofluorescent microscope analysis of Hela cells using anti-BAP37 polyclonal antibody (red). Nuclei were stain with DAPI (blue).
Prohibitin 2 / PHB2 Antibody - MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Prohibitin 2 / PHB2 Antibody - Anti-PHB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Prohibitin 2 / PHB2 Antibody - Immunofluorescent microscope analysis of Hela cells using anti-BAP37 polyclonal antibody (red). Nuclei were stain with DAPI (blue).
Prohibitin 2 / PHB2 Antibody - MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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IHC‑plus™ Polyclonal Rabbit anti‑Mouse Prohibitin 2 / PHB2 Antibody (C‑Terminus, IHC, WB) LS‑B1889

IHC‑plus™ Polyclonal Rabbit anti‑Mouse Prohibitin 2 / PHB2 Antibody (C‑Terminus, IHC, WB) LS‑B1889

Note: This antibody replaces LS-C41304
Antibody:
Prohibitin 2 / PHB2 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B1889-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Prohibitin 2 / PHB2 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
PHB2 antibody LS-B1889 is an unconjugated rabbit polyclonal antibody to PHB2 (Prohibitin 2) (C-Terminus) from mouse. It is reactive with human and mouse. Validated for ICC, IHC and WB. Cited in 2 publications.
Target
Mouse Prohibitin 2 / PHB2
Synonyms
PHB2 | BCAP37 | BAP | D-prohibitin | p22 | Prohibitin-2 | B-cell associated protein | Bap37 | PNAS-141 | Prohibitin 2 | REA
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant mouse PHB2.
Epitope
C-Terminus
Specificity
Recombinant (partial), C-terminal.
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1889 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1889 was determined to be 1:50.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Prohibitin 2 / PHB2
Q99623 NM_007273 NP_001138303.1

Validation

Prohibitin 2 / PHB2 Antibody - Anti-PHB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-PHB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-PHB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

LSBio Ratings

IHC-plus™ Prohibitin 2 / PHB2 Antibody (C-Terminus) for IHC, ICC, WB/Western LS-B1889 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Identification of the cellular prohibitin 1/prohibitin 2 heterodimer as an interaction partner of the C-terminal cytoplasmic domain of the HIV-1 glycoprotein. Emerson V, Holtkotte D, Pfeiffer T, Wang IH, Schnlzer M, Kempf T, Bosch V. Journal of virology. 2010 84:1355-65.

Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. Lucas CR, Cordero-Nieves HM, Erbe RS, McAlees JW, Bhatia S, Hodes RJ, Campbell KS, Sanders VM. Journal of immunology (Baltimore, Md. : 1950). 2013 190:723-36.

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Requested From: United States
Date Requested: 11/12/2024