Human Prohibitin 2 / PHB2

Rabbit

Human, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

His6-tagged fusion protein corresponding to aa88-299 of human REA-1. The immunizing sequence is identical in mouse.

aa88-299

Recognizes mouse REA, Mr 37kD. Species cross-reactivity: human and rat.

• IHC
• IHC - Paraffin (5 µg/ml)
• ICC
• Western blot (0.5 - 2 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B2316 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2316 was determined to be 5 ug/ml. Immunocytochemistry. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates REA from MCF-7 and 3T3 whole cell extracts. Immunocytochemistry: Nuclear staining of REA in paraformaldehyde fixed, Triton X-100 permeabilized MCF-7 cells.

Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99623 NCBI: NM_007273 NP_001138303.1