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Catalog Number Size Price
LS-C330792-20 20 µl $275 
LS-C330792-100 100 µl (1.99 mg/ml) $389 
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded human kidney cancer using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunofluorescence analysis of HeLa cells using PRF1 antibody. Blue: DAPI for nuclear staining.
PRF1 / Perforin Antibody - Western blot analysis of extracts of various cell lines, using PRF1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
PRF1 / Perforin Antibody - Western blot analysis of extracts of various cell lines, using PRF1 antibody.
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded human kidney cancer using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunohistochemistry of paraffin-embedded rat kidney using PRF1 antibody at dilution of 1:200 (x400 lens).
PRF1 / Perforin Antibody - Immunofluorescence analysis of HeLa cells using PRF1 antibody. Blue: DAPI for nuclear staining.
PRF1 / Perforin Antibody - Western blot analysis of extracts of various cell lines, using PRF1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
PRF1 / Perforin Antibody - Western blot analysis of extracts of various cell lines, using PRF1 antibody.
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Polyclonal Rabbit anti‑Human PRF1 / Perforin Antibody (IHC, IF, WB) LS‑C330792

Polyclonal Rabbit anti‑Human PRF1 / Perforin Antibody (IHC, IF, WB) LS‑C330792

Antibody:
PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C330792-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PRF1 / Perforin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Perforin antibody LS-C330792 is an unconjugated rabbit polyclonal antibody to Perforin (PRF1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human PRF1 / Perforin
Synonyms
PRF1 | HPLH2 | Perforin | p1 | Perforin1 | PFP | Cytolysin | FLH2 | Perforin-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human PRF1 (NP_001076585.1). EEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW
Specificity
Human PRF1 / Perforin
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 61kDa, while the observed MW by Western blot was 70kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRF1 / Perforin
P14222 NM_005041 NP_005032.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024