Target
Human POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Reactivity
Human, Monkey, Mouse, Horse
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Rat, Bovine, Dog, Mink, Pig, Macaque (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Specificity
This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
Applications
- Immunofluorescence
- Western blot (1:1000 - 1:1500)
- Immunoprecipitation
- ELISA (1:350000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~39 kD in size corresponding to Oct-4 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting endogenous Oct-4.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee