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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1194-100 | 100 µl (1 mg/ml) | $460 |
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Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa114‑163, IHC, WB) LS‑B1194
Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa114‑163, IHC, WB) LS‑B1194
Note: This antibody replaces LS-C30867
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
OCT4 antibody LS-B1194 is an unconjugated rabbit polyclonal antibody to human OCT4 (POU5F1) (aa114-163). Validated for IHC and WB. Cited in 1 publication.
Target
Human POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%); Marmoset (92%); Dog (91%); Bovine, Pig, Guinea pig (85%); Horse (84%); Cat (83%).
Epitope
aa114-163
Specificity
Human POU5F1 / OCT4
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1.25 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1194 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1194 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About POU5F1 / OCT4
LSBio Ratings
POU5F1 / OCT4 Antibody (aa114-163) for IHC, WB/Western LS-B1194 has an LSBio Rating of
Publications (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species. Sho Yoshimatsu, Atsushi Yamazaki, Kazuya Edamura, Yuko Koushige, Hisashi Shibuya, Emi Qian, Tsukika Sato, Junko Okahara, Noriyuki Kishi, Toshiaki Noce, Yoshifumi Yamaguchi, Hideyuki Okano. Development, growth & differentiation. 2022 August;64:325-341.
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
