Target
Mouse POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DRPNAVKLEKVEP, from the internal region of the protein sequence according to NP_038661.2;.
Specificity
Mouse POU5F1 / OCT4.
Applications
- Western blot (1 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 40kD band observed in lysates of cell line NIH3T3 (calculated MW of 38.2kD according to NP_038661.2). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee