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Antibodies > PDPK1 / PDK1 > LS-C800879
 
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Catalog Number Size Price
LS-C800879-100 100 µl (1 mg/ml) $379 
LS-C800879-200 200 µl (1 mg/ml) $421 
PDPK1 / PDK1 Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human TB tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of mouse brain tissue using Phospho-PDK1 (Ser241) antibody.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in EGF treated MDA-MB-435 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human TB tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of mouse brain tissue using Phospho-PDK1 (Ser241) antibody.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in EGF treated MDA-MB-435 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (phospho‑Ser241, IHC, WB) LS‑C800879

Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (phospho‑Ser241, IHC, WB) LS‑C800879

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Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal (pSer241) Antibody
Application:
IHC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800879-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal (pSer241) Antibody
Application:
IHC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PDK1 antibody LS-C800879 is an unconjugated rabbit polyclonal antibody to PDK1 (PDPK1) (pSer241) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Target
Human PDPK1 / PDK1
Synonyms
PDPK1 | HPDK1 | PkB kinase like gene 1 | PkB-like 1 | Pdk-1 | PkB kinase | PRO0461
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PDK1 around the phosphorylation site of Serine 241.
Epitope
pSer241
Specificity
Phospho-PDK1 (Ser241) Antibody detects endogenous levels of PDK1 only when phosphorylated at Serine 241.
Applications
  • IHC (1:50 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDPK1 / PDK1
O15530 NM_002613 NP_002604.1

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Applications: Western blot, Immunoprecipitation
PDPK1 / PDK1 Antibody - Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PDPK1 / PDK1 Antibody (phospho‑Ser241) LS‑C352641
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See all PDPK1 / PDK1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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customerservice@vectorlabs.com

For questions about orders:
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