Human PDPK1 / PDK1

Mouse

IgG2a Monoclonal

Unconjugated

Ascites

Unmodified

Synthetic peptides corresponding to the PDK1 docking motif, followed by extensive screening and characterization of hybridoma clones (KLH). Motif: (FK)XX(F/Y)(S*/T*)(F/Y).

pSer/Thr

Detects phosphorylated Ser or Thr that is surrounded by Tyr or Phe at the -1 and +1 positions and Phe at the -4 position. It also recognizes peptides containing Lys instead of Phe at the -4 position. Does not cross-react with the non-phosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt.

• Western blot (1:1000)
• Immunoprecipitation (1:20)
• ELISA (1:2000)

Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:20. ELISA (Peptide): 1:1000.

10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.2% sodium azide, 50% glycerol.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15530 NCBI: NM_002613 NP_002604.1