Human PD-L2 / PDCD1LG2 / CD273

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

ANC8D12

Unconjugated. Also available conjugated with Biotin, FITC.

Protein A purified

Unmodified

Recombinant human CD273(B7-DC).

Antibody ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.

• Flow Cytometry
• ELISA
• Blocking

Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-human CD273 antibody at concentration of 5 ug/ml. They were then washed twice and incubated with Goat-anti-Mouse/FITC, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to isotype control Mouse IgG2a antibody. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273- muIg.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BQ51 NCBI: NM_025239 NP_079515.2