Human PD-L2 / PDCD1LG2 / CD273

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

ANC8D12

Biotin. Also available Unconjugated or conjugated with FITC.

Protein A purified

Unmodified

Recombinant human CD273(B7-DC).

Antibody <span style="font-size: 11.0pt; mso-bidi-font-size: 10.0pt;">ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.

• Flow Cytometry
• ELISA
• Blocking
• Applications tested for the base form of this product only

Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of antihuman CD273/Biotin at concentration of 10 ug/ml. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9BQ51 NCBI: NM_025239 NP_079515.2