Target
Human NGFR / CD271 / TNR16
Synonyms
NGFR | CD271 | CD271 antigen | gp80-LNGFR | Nerve growth factor receptor | p75 ICD | p75(NTR) | p75NTR | TNFR superfamily, member 16 | NGF receptor | TNFRSF16 | p75-NTR
Reactivity
Human, Rat, Hamster, Horse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-PEGSDSTAPSTQEP, from the internal region of the protein sequence according to NP_002498.1.
Specificity
Human NGFR. This antibody is expected to recognize the extracellular domain of p75NTR.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 75kD band observed in Rat Heart lysates (calculated MW of 45.2kD according to Human NP_002498.1 and 45.4kD according to Rat NP_036742.2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1):49-57). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NGFR / CD271 / TNR16