Target
Rat NGFR / CD271 / TNR16
Synonyms
NGFR | CD271 | CD271 antigen | gp80-LNGFR | Nerve growth factor receptor | p75 ICD | p75(NTR) | p75NTR | TNFR superfamily, member 16 | NGF receptor | TNFRSF16 | p75-NTR
Reactivity
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
GST fusion protein corresponding to the intracellular domain (aa 274-425) of rat p75 neurotrophin receptor.
Specificity
Recognizes p75 neurotrophin receptor, Mr ~75kD. Species cross-reactivity: Rat, human and mouse.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.1 - 5 µg/ml)
- Immunoprecipitation
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Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.1-05 ug/ml detected p75 neurotrophin receptor in RIPA lysates from PC-12 cells. PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p75 Neurotrophin Receptor (0.1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate p75 neurotrophin receptor. Immunohistochemistry: Reported to detect p75 neurotrophin receptor in rat brain sections.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NGFR / CD271 / TNR16