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Antibodies > NEFM / NF-M > LS-C354909
 
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Catalog Number Size Price
LS-C354909-100 100 µg (0.5 mg/ml) $504 
NEFM / NF-M Antibody - Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
NEFM / NF-M Antibody - Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament light chain monoclonal antibody in 0.3% BSA at a dilution of 1:50 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
NEFM / NF-M Antibody - Western blot analysis of neurofilament, medium chain was performed by loading 25ug of mouse brain tissue lysate per well onto a polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked. NF-M was detected at ~160kD using a neurofilament, medium chain antibody at a dilution of 2 µg/mL in blocking buffer overnight at 4C, followed by a HRP-labeled secondary antibody for 1 hour at room temperature and detection with a chemiluminescent substrate.
NEFM / NF-M Antibody - Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
NEFM / NF-M Antibody - Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament light chain monoclonal antibody in 0.3% BSA at a dilution of 1:50 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
NEFM / NF-M Antibody - Western blot analysis of neurofilament, medium chain was performed by loading 25ug of mouse brain tissue lysate per well onto a polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked. NF-M was detected at ~160kD using a neurofilament, medium chain antibody at a dilution of 2 µg/mL in blocking buffer overnight at 4C, followed by a HRP-labeled secondary antibody for 1 hour at room temperature and detection with a chemiluminescent substrate.
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Monoclonal Mouse anti‑Rat NEFM / NF‑M Antibody (clone RMO 14.9, IHC, IF, WB) LS‑C354909

Monoclonal Mouse anti‑Rat NEFM / NF‑M Antibody (clone RMO 14.9, IHC, IF, WB) LS‑C354909

Submit Review LSBio Guarantee
Antibody:
NEFM / NF-M Mouse anti-Rat Monoclonal (RMO 14.9) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Rat, Human, Mouse, Rabbit, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$504
LS-C354909-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NEFM / NF-M Mouse anti-Rat Monoclonal (RMO 14.9) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Rat, Human, Mouse, Rabbit, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
NF-M antibody LS-C354909 is an unconjugated mouse monoclonal antibody to NF-M (NEFM) from rat. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Rat NEFM / NF-M
Synonyms
NEFM | 160 kDa neurofilament protein | NEF3 | NF-M | NFM | Neurofilament 3
Host
Mouse
Reactivity
Rat, Human, Mouse, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
RMO 14.9
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Rat NF-M
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFM / NF-M
P07197 NM_005382 NP_005373.2

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Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF403) LS‑B7183
Species: Human, Monkey, Hamster, Xenopus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF406) LS‑B7184
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF‑M Antibody (aa413‑773, clone OTI5B7) LS‑C339771
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
NEFM / NF-M ELISA Kit
Human NEFM / NF-M (Sandwich ELISA) ELISA Kit - LS-F4561
Reactivity: Human
Range: 15.63-1000 pg/ml
See all NEFM / NF-M Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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