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Antibodies > NEFM / NF-M > LS-C339771
 
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Catalog Number Size Price
LS-C339771-100 100 µl (1 mg/ml) $434 
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human muscle tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human testicular cancer tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic cerebellum using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic liver tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human adult heart tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human melanoma tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human adult brain tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human muscle tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human testicular cancer tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic cerebellum using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human embryonic liver tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human adult heart tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human melanoma tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - IHC of paraffin-embedded Human adult brain tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
NEFM / NF-M Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM.
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Monoclonal Mouse anti‑Human NEFM / NF‑M Antibody (clone OTI5B7, aa413‑773, IHC, WB) LS‑C339771

Monoclonal Mouse anti‑Human NEFM / NF‑M Antibody (clone OTI5B7, aa413‑773, IHC, WB) LS‑C339771

Submit Review LSBio Guarantee
Antibody:
NEFM / NF-M Mouse anti-Human Monoclonal (aa413-773) (OTI5B7) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Carrier-free
Price
Catalog Number
$434
LS-C339771-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NEFM / NF-M Mouse anti-Human Monoclonal (aa413-773) (OTI5B7) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Carrier-free

Specifications

Description
NF-M antibody LS-C339771 is an unconjugated mouse monoclonal antibody to human NF-M (NEFM) (aa413-773). Validated for IHC and WB.
Target
Human NEFM / NF-M
Synonyms
NEFM | 160 kDa neurofilament protein | NEF3 | NF-M | NFM | Neurofilament 3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI5B7
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified. Also available Carrier-free.
Immunogen
Human recombinant protein fragment corresponding to amino acids 413-773 of human NEFM(NP_005373) produced in E. coli.
Epitope
aa413-773
Specificity
Human NEFM / NF-M
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFM / NF-M
P07197 NM_005382 NP_005373.2

Publications (0)

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Featured Products

NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (aa250‑263) LS‑B11174
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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NEFM / NF‑M Antibody LS‑C16790
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NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF403) LS‑B7183
Species: Human, Monkey, Hamster, Xenopus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
NEFM / NF-M Antibody - Anti-NEFM / NF-M antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ NEFM / NF‑M Antibody (clone RNF406) LS‑B7184
Species: Human, Monkey, Guinea pig, Hamster, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
NEFM / NF-M Antibody - Immunohistochemistry analysis of the neurofilament medium chain showing staining in the filaments of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament medium chain monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
NEFM / NF‑M Antibody (clone RMO 14.9) LS‑C354909
Species: Rat, Human, Mouse, Rabbit, Chicken
Applications: IHC, Immunofluorescence, Western blot, ELISA
NEFM / NF-M ELISA Kit
Human NEFM / NF-M (Sandwich ELISA) ELISA Kit - LS-F4561
Reactivity: Human
Range: 15.63-1000 pg/ml
See all NEFM / NF-M Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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United States

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