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Antibodies > MYB / c-Myb > LS-C801551
 
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Catalog Number Size Price
LS-C801551-100 100 µl (1 mg/ml) $379 
LS-C801551-200 200 µl (1 mg/ml) $421 
MYB / c-Myb Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
MYB / c-Myb Antibody - Western blot analysis of Myb expression in 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
MYB / c-Myb Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
MYB / c-Myb Antibody - Western blot analysis of Myb expression in 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human MYB / c‑Myb Antibody (IF, WB) LS‑C801551

Polyclonal Rabbit anti‑Human MYB / c‑Myb Antibody (IF, WB) LS‑C801551

Submit Review LSBio Guarantee
Antibody:
MYB / c-Myb Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801551-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MYB / c-Myb Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C-Myb antibody LS-C801551 is an unconjugated rabbit polyclonal antibody to c-Myb (MYB) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human MYB / c-Myb
Synonyms
MYB | C-myb_CDS | C-myb8B_CDS | C-myb protein (140 AA) | C-myb10A_CDS | C-myb13A_CDS | Efg | Transcriptional activator Myb | C-myb | C-myb14A_CDS | Cmyb | Proto-oncogene c-Myb
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Myb.
Specificity
Myb Antibody detects endogenous levels of total Myb.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYB / c-Myb
P10242 NM_005375 NP_005366.2

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Featured Products

MYB / c-Myb Antibody - Anti-c-Myb antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ MYB / c‑Myb Antibody (aa1‑50) LS‑B5315
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
MYB / c-Myb Antibody - Anti-C-Myb Antibody - Western Blot. Western blot of affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kD corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45mum membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Personal communication, J. Bies, NCI NIH, Bethesda.
MYB / c‑Myb Antibody (Internal) LS‑C153812
Species: Mouse
Applications: Western blot, ELISA
Product
MYB / c‑Myb Antibody (aa119‑135, clone MYB286) LS‑C391542
Species: Human
Applications: Flow Cytometry
MYB / c-Myb Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ MYB / c‑Myb Antibody (N‑Terminus) LS‑B14085
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
MYB / c-Myb Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
MYB / c‑Myb Antibody (aa26‑75) LS‑B11880
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
MYB / c-Myb Antibody - Goat anti-MYB / c-myb Antibody (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
MYB / c‑Myb Antibody (aa281‑294) LS‑C55135
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all MYB / c-Myb Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
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Tel: (800) 227-6666 / (650) 697-3600

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