Target
Human MSLN / Mesothelin
Synonyms
MSLN | CAK1 antigen | MPF | SMRP | Mesothelin | CAK1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CPSGKKAREIDES, from the internal region of the protein sequence according to NP_005814.2NP_037536.2.
Specificity
Human MSLN / Mesothelin. This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 68.1kDa according to NP_005814.2 and of 69.0kDa according to NP_037536.2). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee