Target
Human MSLN / Mesothelin
Synonyms
MSLN | CAK1 antigen | MPF | SMRP | Mesothelin | CAK1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived recombinant human mesothelin.
Specificity
Recognizes human mesothelin. This antibody binds to mesothelin cleaved form which is the ~40 kDa cell membrane-associated glycosylphosphatidylinositol-linked glycoprotein. It is not expected to react with the soluble portion of the mesothelin precursor protein that is designated as the megakaryocyte potentiating factor.
Applications
- Flow Cytometry (2.5 µg/10E6 cells)
- ELISA (0.5 - 1 µg/ml)
Usage
Flow Cytometry: This antibody was validated for flow cytometry using Hela human cervical carcinoma cells. Use at 2.5 ug per 10e6 cells. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Mesothelin. The detection limit for rhMesothelin is approximately 5 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee