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Catalog Number Size Price
LS-C662634-10 10 µg $318 
LS-C662634-100 100 µg $470 
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Western blot analysis of MMP13 using anti-MMP13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54KD. The expected band size for MMP13 is at 54KD.
MMP13 Antibody - Western blot - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Immunohistochemistry - Anti-MMP13 Picoband antibody
MMP13 Antibody - Western blot analysis of MMP13 using anti-MMP13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP13 at approximately 54KD. The expected band size for MMP13 is at 54KD.
MMP13 Antibody - Western blot - Anti-MMP13 Picoband antibody
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Polyclonal Rabbit anti‑Rat MMP13 Antibody (IHC, WB) LS‑C662634

Polyclonal Rabbit anti‑Rat MMP13 Antibody (IHC, WB) LS‑C662634

Antibody:
MMP13 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662634-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MMP13 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MMP13 antibody LS-C662634 is an unconjugated rabbit polyclonal antibody to rat MMP13. Validated for ELISA, IHC and WB.
Target
Rat MMP13
Synonyms
MMP13 | Collagenase 3 | Matrix metalloproteinase-13 | Interstitial collagenase | MANDP1 | MMP-13 | CLG3
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MMP13
P45452 NM_002427 NP_002418.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/29/2024