Target
Pig ME1 / Malate Dehydrogenase
Synonyms
ME1 | HUMNDME | Malate dehydrogenase | Malic enzyme 1 | Malate oxidoreductase | Malic enzyme 1, soluble | MES | NADP-ME | Pyruvic-malic carboxylase | NADP-dependent malic enzyme | Malic enzyme, cytoplasmic
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Malate Dehydrogenase [Pig Heart].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.
Applications
- IHC (1:50 - 1:250)
- Western blot (1:1000 - 1:5000)
- Immunoprecipitation (1:50)
- ELISA (1:1000 - 1:5000)
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Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ME1 / Malate Dehydrogenase