Target
Pig ME1 / Malate Dehydrogenase
Synonyms
ME1 | HUMNDME | Malate dehydrogenase | Malic enzyme 1 | Malate oxidoreductase | Malic enzyme 1, soluble | MES | NADP-ME | Pyruvic-malic carboxylase | NADP-dependent malic enzyme | Malic enzyme, cytoplasmic
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
Malate Dehydrogenase [Pig Heart].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources is unknown.
Applications
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ME1 / Malate Dehydrogenase