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Catalog Number	Size	Price
LS-C354039-100	100 µl (1 mg/ml)	$315

MDM2 Antibody - Immunohistochemical analysis of MDM2 (pS166) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

MDM2 Antibody - Immunofluorescent analysis of MDM2 (pS166) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MDM2 Antibody - Western blot analysis of MDM2 (pS166) expression in HEK293T hydroxyurea-treated (A); MCF7 IGF-treated (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human MDM2 Antibody (phospho‑Ser166, IHC, IF, WB) LS‑C354039

Name: Anti-MDM2 Antibody | Rabbit anti-Human Polyclonal ICC,IF,IHC,IP,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C354039-100
Price: 315.0000 USD
Availability: InStock

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Antibody:

MDM2 Rabbit anti-Human Polyclonal (pSer166) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

$315

LS-C354039-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MDM2 Rabbit anti-Human Polyclonal (pSer166) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

MDM2 antibody LS-C354039 is an unconjugated rabbit polyclonal antibody to human MDM2 (pSer166). Validated for ICC, IF, IHC, IP and WB.

Target

Human MDM2

Synonyms

See All MDM2 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human MDM2 (pS166).

Epitope

pSer166

Specificity

Recognizes endogenous levels of MDM2 (pS166) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MDM2

Uniprot: Q00987 NCBI: NM_002392 NP_002383.2