Target
Human MDM2
Synonyms
MDM2 | ACTFS | Double minute 2 protein | Hdm2 | MDM2 variant FB30 | HDMX | Oncoprotein Mdm2 | p53-binding protein Mdm2 | MDM2 variant FB28
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
A synthetic peptide derived from human MDM2 protein (1).
Specificity
Recognizes human MDM2 protein. Does not react with mouse MDM2. Epitope recognized by this antibody is located within amino acids 26-169 of the human protein.
Applications
- IHC
- IHC - Paraffin (1 - 5 µg/ml)
- IHC - Frozen (1 - 5 µg/ml)
- Immunofluorescence (1 - 5 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 ug/ml. Western Blot: 0.5-2 ug/ml (chemiluminescence detection). Immunohistochemistry (frozen): 1-5 ug/ml. Immunohistochemistry (formalin-fixed, paraffin-embedded): 1-5 ug/ml. Staining requires heat induced epitope retrieval (HIER) pretreatment. Immunoprecipitation: 1 ug/sample (autoradiographic detection). Positive control: OsA-CL, MCF-7 and HeLa cell lysates.
Presentation
PBS, pH 7.4, 0.05% sodium azide, 1 mg/ml BSA, 30% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee