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Catalog Number	Size	Price
LS-C168823-400	400 µl	$393

MAP1LC3B / LC3B Antibody - SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

MAP1LC3B / LC3B Antibody - Time course study of mouse leukemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.

MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa89‑122, IHC, IF) LS‑C168823

Name: Anti-MAP1LC3B / LC3B Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C168823-400
Price: 393.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa89-122) Antibody

Application:

IHC, ICC, IF

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$393

LS-C168823-400

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For Research Use Only

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Overview

Antibody:

MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa89-122) Antibody

Application:

IHC, ICC, IF

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

LC3B antibody LS-C168823 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa89-122) from human. It is reactive with human and mouse. Validated for ICC, IF and IHC.

Target

Human MAP1LC3B / LC3B

Synonyms

See All MAP1LC3B / LC3B Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa89-122

Specificity

This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Applications

IHC
ICC (1:10 - 1:50)
Immunofluorescence (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP1LC3B / LC3B

Uniprot: Q9GZQ8 NCBI: NM_022818 NP_073729.1