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Catalog Number	Size	Price
LS-B9581-200	200 µl	$460

MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

MAP1LC3B / LC3B Antibody - APG8a/b (MAP1LC3A/B) western blot of mouse lung tissue lysates (35 ug/lane). The MAP1LC3A antibody detected the MAP1LC3A protein (arrow).

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IHC‑plus™ Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa100‑125, IHC, IF, WB) LS‑B9581

Name: IHC-plus™ MAP1LC3B / LC3B Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B9581-200
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C156409

Antibody:

MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa100-125) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$460

LS-B9581-200

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For Research Use Only

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Overview

Antibody:

MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa100-125) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

LC3B antibody LS-B9581 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa100-125) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human MAP1LC3B / LC3B

Synonyms

See All MAP1LC3B / LC3B Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa100-125

Specificity

This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:100)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP1LC3B / LC3B

Uniprot: Q9GZQ8 NCBI: NM_022818 NP_073729.1

Validation

Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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About LSBio Validation process