Macrophage

Mouse

Human (tested or 100% immunogen sequence identity)

Monoclonal

PM-2K

Unconjugated

Affinity purified

Unmodified

Cultured human peritoneal macrophages

The antigen of 150kDa m.w. is found in the macrophage cell membrane. The epitope has not been determined. Antigen distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages and macrophages in the interstitial tissues of the kidney, pancreas and many other organs. In Gaucher's disease multicentric reticulohistiocytosis and malignant histiocytosis proliferating macrophages are positive. In MFH infiltrating macrophages are stained but not tumour cells. Some bone stromal cells but not osteoclast-like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are negative. Specificity: Human: Macrophages. Other: positive on macrophages of cat, dog, pig, bovine and monkey; negative in rabbit, rat, guinea pig, gold hamster, goat, horse.

• IHC
• IHC - Frozen (1:400)

Lyophilized from PBS pH 7.2, 5mg/ml BSA, and 0.05% Kathon CG

Reconstitute with 0.5ml distilled H2O.

Store at 4°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.