Reactivity
Pig
(tested or 100% immunogen sequence identity)
Immunogen
Porcine alveolar macrophages.
Specificity
Recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by the antibody has a broad tissue distribution. Clone BA4D5 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyers patches. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.