Target
Human LTBR
Synonyms
LTBR | D12S370 | CD18 | LT-BETA-R | Lymphotoxin B receptor | Lymphotoxin-beta receptor | TNFR3 | TNF-R-III | TNF-RIII | TNFCR | TNFRSF3 | TNFR-III | TNFR-RP | TNFR2-RP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin,
RPE.
Purification
Protein A purified
Immunogen
Recombinant Human LT.
Specificity
Human LTbR present on U-937 cell surface, and to recombinant LTbR-muIg in EIA.
Applications
- Flow Cytometry
- ELISA
- Applications tested for the base form of this product only
Usage
Five x 10e5 cultured human U-937 cells were pre incubated with 20 ul of 150ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of antibody LS-C134619 at a 1:50 dilution (10 ?g/ml). They were then washed three times, fixed and analyzed by FACS.Or the antibody can be used at 1.6ul of concentrated stock per 5 x 10e5-cell test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee