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Catalog Number Size Price
LS-C352452-100 100 µl (1 mg/ml) $299 
LIG4 / DNA Ligase IV Antibody - Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LIG4 / DNA Ligase IV Antibody - Immunofluorescent analysis of DNA Ligase 4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LIG4 / DNA Ligase IV Antibody - Western blot analysis of DNA Ligase 4 expression in HepG2 (A) whole cell lysates.
LIG4 / DNA Ligase IV Antibody - Immunohistochemical analysis of DNA Ligase 4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LIG4 / DNA Ligase IV Antibody - Immunofluorescent analysis of DNA Ligase 4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
LIG4 / DNA Ligase IV Antibody - Western blot analysis of DNA Ligase 4 expression in HepG2 (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (Internal, IHC, IF, WB) LS‑C352452

Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (Internal, IHC, IF, WB) LS‑C352452

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352452-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Ligase IV antibody LS-C352452 is an unconjugated rabbit polyclonal antibody to DNA Ligase IV (LIG4) (Internal) from human. It is reactive with human, bovine and dog. Validated for ICC, IF, IHC and WB.
Target
Human LIG4 / DNA Ligase IV
Synonyms
LIG4 | DNA ligase IV | DNA joinase | DNA ligase 4 | DNA repair enzyme | Ligase IV, DNA, ATP-dependent | Sealase | Polynucleotide ligase
Host
Rabbit
Reactivity
Human, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human DNA Ligase 4.
Epitope
Internal
Specificity
Recognizes endogenous levels of DNA Ligase 4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG4 / DNA Ligase IV
P49917 NM_002312 NP_002303.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024