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Catalog Number Size Price
LS-C331665-20 20 µl $275 
LS-C331665-100 100 µl (2.54 mg/ml) $389 
LIG4 / DNA Ligase IV Antibody - Western blot analysis of extracts of various cell lines, using LIG4 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (IHC, WB) LS‑C331665

Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (IHC, WB) LS‑C331665

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331665-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Ligase IV antibody LS-C331665 is an unconjugated rabbit polyclonal antibody to DNA Ligase IV (LIG4) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human LIG4 / DNA Ligase IV
Synonyms
LIG4 | DNA ligase IV | DNA joinase | DNA ligase 4 | DNA repair enzyme | Ligase IV, DNA, ATP-dependent | Sealase | Polynucleotide ligase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human LIG4 (NP_002303.2). SKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
Specificity
Human LIG4 / DNA Ligase IV
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 103kDa, while the observed MW by Western blot was 108kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG4 / DNA Ligase IV
P49917 NM_002312 NP_002303.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024