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Catalog Number Size Price
LS-C804697-100 100 µl (1 mg/ml) $379 
LS-C804697-200 200 µl (1 mg/ml) $421 
LIG4 / DNA Ligase IV Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
LIG4 / DNA Ligase IV Antibody - Western blot analysis of extracts of Jurkat cells using DNL4 antibody.
LIG4 / DNA Ligase IV Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
LIG4 / DNA Ligase IV Antibody - Western blot analysis of extracts of Jurkat cells using DNL4 antibody.
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Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (IF, WB) LS‑C804697

Polyclonal Rabbit anti‑Human LIG4 / DNA Ligase IV Antibody (IF, WB) LS‑C804697

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804697-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LIG4 / DNA Ligase IV Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Ligase IV antibody LS-C804697 is an unconjugated rabbit polyclonal antibody to human DNA Ligase IV (LIG4). Validated for IF, Peptide-ELISA and WB.
Target
Human LIG4 / DNA Ligase IV
Synonyms
LIG4 | DNA ligase IV | DNA joinase | DNA ligase 4 | DNA repair enzyme | Ligase IV, DNA, ATP-dependent | Sealase | Polynucleotide ligase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIG4 / DNA Ligase IV
P49917 NM_002312 NP_002303.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/11/2024