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Catalog Number	Size	Price
LS-C359469-0.1	0.1 mg (1 mg/ml)	$386

LIF Antibody - Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LIF Antibody - Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LIF Antibody - Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LIF Antibody - Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). This image was taken for the unconjugated form of this product. Other forms have not been tested.

LIF Antibody - Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunopositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands whereas the surrounding fibromuscular stroma cells did not develop any staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LIF Antibody - Western Blot: LIF Antibody (39N7D10) - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this assay. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Monoclonal Rat anti‑Mouse LIF Antibody (clone 39N7D10, aa24‑203, IHC, WB) LS‑C359469

Name: Anti-LIF Antibody | Rat anti-Mouse Monoclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C359469-0.1
Price: 386.0000 USD
Availability: InStock

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Antibody:

LIF Rat anti-Mouse Monoclonal (aa24-203) (39N7D10) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human

Format:

Unconjugated, Unmodified

$386

LS-C359469-0.1

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

LIF Rat anti-Mouse Monoclonal (aa24-203) (39N7D10) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human

Format:

Unconjugated, Unmodified

Specifications

Description

LIF antibody LS-C359469 is an unconjugated rat monoclonal antibody to LIF (aa24-203) from mouse. It is reactive with human and mouse. Validated for IHC and WB.

Target

Mouse LIF

Synonyms

See All LIF Antibodies

Host

Rat

Reactivity

Mouse, Human (tested or 100% immunogen sequence identity)

Clonality

IgG2b,k Monoclonal

Clone

39N7D10

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Immunogen

A recombinant murine Lif protein containing amino acids 24-203.

Epitope

aa24-203

Specificity

Mouse LIF

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot (3 - 5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target).

Presentation

PBS, 0.05% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About LIF

Uniprot: P15018 NCBI: NM_002309 NP_002300.1