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Catalog Number	Size	Price
LS-C313429-10	10 µg	$318
LS-C313429-100	100 µg (0.5 mg/ml)	$470

LIF Antibody - Immunohistochemistry - Anti-LIF Picoband Antibody

LIF Antibody - Western blot analysis of LIF using anti-LIF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LIF at approximately 36KD. The expected band size for LIF is at 22KD.

LIF Antibody - Western blot - Anti-LIF Picoband Antibody

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Polyclonal Rabbit anti‑Human LIF Antibody (aa23‑202, IHC, WB) LS‑C313429

Name: Anti-LIF Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313429-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

LIF Rabbit anti-Human Polyclonal (aa23-202) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C313429-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

LIF Rabbit anti-Human Polyclonal (aa23-202) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

LIF antibody LS-C313429 is an unconjugated rabbit polyclonal antibody to human LIF (aa23-202). Validated for IHC and WB.

Target

Human LIF

Synonyms

See All LIF Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.

Epitope

aa23-202

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About LIF

Uniprot: P15018 NCBI: NM_002309 NP_002300.1