IHC‑plus™ Monoclonal Mouse anti‑Human KMT2A / MLL Antibody (clone 10F8D7, aa3751‑3968, IHC, WB) LS‑B3471
IHC‑plus™ Monoclonal Mouse anti‑Human KMT2A / MLL Antibody (clone 10F8D7, aa3751‑3968, IHC, WB) LS‑B3471
Note: This antibody replaces LS-C41523, LS-C355338, LS-B7346
Antibody:
KMT2A / MLL Mouse anti-Human Monoclonal (aa3751-3968) (10F8D7) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
KMT2A / MLL Mouse anti-Human Monoclonal (aa3751-3968) (10F8D7) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
MLL antibody LS-B3471 is an unconjugated mouse monoclonal antibody to human MLL (KMT2A) (aa3751-3968). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KMT2A / MLL
Synonyms
KMT2A | ALL1 | CDK6/MLL fusion protein | FLJ11783 | HRX | HTRX | HTRX1 | Lysine N-methyltransferase 2A | MLL1A | MLL/GAS7 | MLL1 | ALL-1 | MLL | MLL/GAS7 fusion protein | TRX1 | WDSTS | Trithorax-like protein | Zinc finger protein HRX | CXXC7 | MLL-AF4 der(11) fusion protein | MLL/GMPS fusion protein | TET1-MLL
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Purified recombinant fragment of MLL (aa3751-3968) expressed in E. coli.
Specificity
Purified truncated recombinant MLL-Trx(aa3751-3968) is expressed in E. coli strain BL21 (DE3).
Applications
- IHC
- IHC - Paraffin (1:200)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3471 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3471 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-MLL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MLL antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse
Applications:
IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
Western blot, Immunoprecipitation
Species:
Human
Applications:
Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Species:
Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/3/2025