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Catalog Number	Size	Price
LS-C662738-10	10 µg	$318
LS-C662738-100	100 µg	$470

ITGA5/Integrin Alpha 5/CD49e Antibody - Immunohistochemistry - Anti-Integrin alpha 5 Picoband antibody

ITGA5/Integrin Alpha 5/CD49e Antibody - IF analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody Integrin alpha 5 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-Integrin alpha 5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

ITGA5/Integrin Alpha 5/CD49e Antibody - Western blot - Anti-Integrin alpha 5 Picoband antibody

ITGA5/Integrin Alpha 5/CD49e Antibody - Flow Cytometry analysis of HT-1080 cells using anti-Integrin alpha 5 antibody. Overlay histogram showing HT-1080 cells stained with anti-Integrin alpha 5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

ITGA5/Integrin Alpha 5/CD49e Antibody - Flow Cytometry analysis of A431 cells using anti-Integrin alpha 5 antibody. Overlay histogram showing A431 cells stained with anti-Integrin alpha 5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human ITGA5/Integrin Alpha 5/CD49e Antibody (IHC, WB) LS‑C662738

Name: Anti-ITGA5/Integrin Alpha 5/CD49e Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662738-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

ITGA5/Integrin Alpha 5/CD49e Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662738-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ITGA5/Integrin Alpha 5/CD49e Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD49e antibody LS-C662738 is an unconjugated rabbit polyclonal antibody to human CD49e (ITGA5 / Integrin Alpha 5). Validated for ELISA, IHC and WB.

Target

Human ITGA5/Integrin Alpha 5/CD49e

Synonyms

See All ITGA5/Integrin Alpha 5/CD49e Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).

Applications

IHC
IHC - Paraffin
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ITGA5/Integrin Alpha 5/CD49e

Uniprot: P08648 NCBI: NM_002205 NP_002196.2