Human ITGA5/Integrin Alpha 5/CD49e

Rabbit

Human, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide derived from the C-Terminus sequence (cytoplasmic domain) of the human alpha 5 integrin subunit.

C-Terminus

Recognizes human Integrin alpha 5. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V.

• IHC
• IHC - Paraffin (1:100)
• ICC (1:1000)
• Western blot (1:1000)
• Immunoprecipitation
• ELISA (1:500 - 1:2000)

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Immunofluorescence and RIA. ELISA: 1:500-1:2000. Western Blot: 1:1000 detects integrin alpha 5 on 10 ug of HUVEC lysates. Use denatured but non-reduced samples to detect full length product. ECL; BSA blocking suggested. Immunoprecipitation: Use 5 ul antibody for 5x1E6 cells. Immunohistochemistry (Acetone fixed tissues): 1:1000. Immunohistochemistry (FFPE tissues): 1:100. Immunocytochemistry: 1:1000.

0.05% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08648 NCBI: NM_002205 NP_002196.2