Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C125831-100 | 100 µg | $919 |
Monoclonal Mouse anti‑Mouse IRS1 Antibody (Mouse pSer307 and Human pSer312, IF, WB) LS‑C125831
Monoclonal Mouse anti‑Mouse IRS1 Antibody (Mouse pSer307 and Human pSer312, IF, WB) LS‑C125831
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
IRS1 antibody LS-C125831 is an unconjugated mouse monoclonal antibody to IRS1 (Mouse pSer307 and Human pSer312) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IF, IP and WB.
Target
Mouse IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Mouse
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Epitope
Mouse pSer307 and Human pSer312
Specificity
Recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human). Species cross-reactivity: rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Applications
- ICC (1:500)
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:1000)
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 1:1000. Immunocytochemistry: 1:500. Immunoprecipitation: 1:1000.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
