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| Catalog Number | Size | Price |
|---|---|---|
| LS-C123905-100 | 100 µg | $880 |
Monoclonal Mouse anti‑Mouse IRS1 Antibody (phospho‑Ser302, IHC, IF, WB) LS‑C123905
Monoclonal Mouse anti‑Mouse IRS1 Antibody (phospho‑Ser302, IHC, IF, WB) LS‑C123905
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
IRS1 antibody LS-C123905 is an unconjugated mouse monoclonal antibody to IRS1 (pSer302) from mouse. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.
Target
Mouse IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Mouse
Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic linear peptide corresponding to amino acids at and around phosphorylated Ser302 of mouse IRS1.
Epitope
pSer302
Specificity
Recognizes IRS1 phosphorylated at Ser302 (mouse) and Ser307 (human). Species cross-reactivity: rat. Species sequence homology: monkey, bovine, canine, porcine. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Applications
- IHC
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Failed Applications
- IHC - Paraffin
Usage
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:1000.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
