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| Catalog Number | Size | Price |
|---|---|---|
| LS-C125829-100 | 100 µg | $880 |
Monoclonal Mouse anti‑Human IRS1 Antibody (aa431‑446, WB) LS‑C125829
Monoclonal Mouse anti‑Human IRS1 Antibody (aa431‑446, WB) LS‑C125829
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
IRS1 antibody LS-C125829 is an unconjugated mouse monoclonal antibody to IRS1 (aa431-446) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IP and WB.
Target
Human IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa431-446 of mouse IRS1. Epitope: Internal.
Epitope
aa431-446
Specificity
Recognizes human IRS1. Species cross-reactivity: mouse and rat. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Applications
- ICC (1:500)
- Western blot (1:1000)
- Immunoprecipitation (1:1000)
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Immunocytochemistry: 1:500. Immunoprecipitation: 1:1000.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
