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Antibodies > IRF3 > LS-C392581
 
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Catalog Number Size Price
LS-C392581-200 200 µl $393 
IRF3 Antibody - Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
IRF3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
IRF3 Antibody - All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IRF3 Antibody - Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
IRF3 Antibody - Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
IRF3 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
IRF3 Antibody - All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IRF3 Antibody - Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human IRF3 Antibody (clone 1522CT766.58.24, IHC, IF, WB) LS‑C392581

Monoclonal Mouse anti‑Human IRF3 Antibody (clone 1522CT766.58.24, IHC, IF, WB) LS‑C392581

Submit Review LSBio Guarantee
Antibody:
IRF3 Mouse anti-Human Monoclonal (1522CT766.58.24) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human, African green monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392581-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IRF3 Mouse anti-Human Monoclonal (1522CT766.58.24) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Peptide-ELISA
Reactivity:
Human, African green monkey
Format:
Unconjugated, Unmodified

Specifications

Description
IRF3 antibody LS-C392581 is an unconjugated mouse monoclonal antibody to IRF3 from human. It is reactive with human and african green monkey. Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Target
Human IRF3
Synonyms
IRF3 | Interferon regulatory factor 3 | IRF-3
Host
Mouse
Reactivity
Human, African green monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1522CT766.58.24
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IRF3
Q14653 NM_001571 NP_001562.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
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