Human IRF3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

3F10

Unconjugated

Protein G purified

Unmodified

Recombinant human IRF3 (108-166aa) purified from E. coli.

aa108-166

Recognizes human interferon regulatory factor 3 (IRF3), a 55kD constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity. IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta, along with other interferon-induced genes.

• Western blot (1:2000 - 1:3000)
• Immunoprecipitation
• ELISA

Western Blot: LS-C45030 detects a band of approximately 55kD in HeLa cell lysates.

PBS, 0.01% Sodium Azide, 10% Glycerol

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14653 NCBI: NM_001571 NP_001562.1