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Catalog Number Size Price
LS-C413182-100 100 µl (1 mg/ml) $341 
IRF3 Antibody - Immunohistochemical analysis of IRF3 (pS385) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IRF3 Antibody - Immunofluorescent analysis of IRF3 (pS385) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IRF3 Antibody - Western blot analysis of IRF3 (pS385) expression in HEK293T (A); HT29 (B) whole cell lysates.
IRF3 Antibody - Immunohistochemical analysis of IRF3 (pS385) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IRF3 Antibody - Immunofluorescent analysis of IRF3 (pS385) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IRF3 Antibody - Western blot analysis of IRF3 (pS385) expression in HEK293T (A); HT29 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human IRF3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C413182

Polyclonal Rabbit anti‑Human IRF3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C413182

Antibody:
IRF3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$341
LS-C413182-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IRF3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
IRF3 antibody LS-C413182 is an unconjugated rabbit polyclonal antibody to IRF3 (C-Terminus) from human. It is reactive with human, mouse, bovine and other species. Validated for ICC, IF, IHC and WB.
Target
Human IRF3
Synonyms
IRF3 | Interferon regulatory factor 3 | IRF-3
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human IRF3 (pS385).
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of IRF3 (pS385) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IRF3
Q14653 NM_001571 NP_001562.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/12/2024