Mouse IL-33

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived rmIL-33.

Recognizes mouse IL-33.

• IHC
• IHC - Frozen (2 - 15 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml. Detection limit for rmIL-33 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 ug/ml Use 10 ul to label 1-5x10^5 cells in a total reaction volume not exceeding 200 ul using activated mouse splenocytes. Immunohistochemistry: 2-15 ug/ml used on frozen mouse intestine samples. Direct ELISA: 0.5-1.0 ug/ml Detection limit for rmIL-33 is ~0.3 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in sterile PBS.

Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95760 NCBI: NM_033439 NP_254274.1